The HT-1080 cell line was taken from the connective tissue of a 35-year-old Caucasian male with fibrosarcoma. HT-1080 cells are tumorigenic and have modal chromosome number of 46. The HT-1080 cell line has epithelial morphological characteristics with culturally adherent properties. It is suitable for transfection, and an HT-1080 transfection reagent is available here through Altogen Biosystems . Transfection of HT-1080 cells can be used to study connective tissue cancers. HT-1080 cells express oncogene N-ras and are susceptible to Human poliovirus 1, Feline leukemia virus, vesicular stomatitis virus, and RD-114 feline virus.